It's a polymorphism that we could use to follow the inheritance of DNA. Separate aliquots of the PCR amplicon are digested with each of up to six different restriction enzymes to produce a species-specific RFLP profile. Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary. That sequence difference doesn't necessarily mean that there's a disease associated with it. Restriction fragment length polymorphism (RFLP) analysis utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA. So these differences in nucleic acid sequences and restriction enzyme binding sites just mean that there's a difference in the sequence between those two people. In one person, without the enzyme site you'll see one band, and the person that has the enzyme site, you'll see two bands, representing the two cleaved products. We typically see these, or we monitor these, by isolating the DNA, cutting it with that bacterial restriction enzyme, and running it on a gel using electrophoresis. And that results in a polymorphism, or difference between those two people. So then, if you isolate that piece of DNA surrounding that site from two people, from one of them it will be cut by the enzyme and the other one it won't. A restriction fragment length polymorphism is said to occur when the length of a detected fragment varies between individuals, indicating non-identical sequence. Restriction fragment length polymorphism (RFLP) was the first molecular marker technique and the only marker system based on hybridization which is one of the. So a single base difference between two people could result in either the presence or absence of that restriction site. So why is that useful? Well, we can take advantage of this fact to actually look for differences between people if they have that restriction enzyme site or not. What is it, though? So basically, if you follow the sequence of DNA, particular sites, a series of four to eight nucleic acids, results in a restriction site where an enzyme from bacteria can actually bind and cleave that DNA. RFLPs have been very useful to use as markers for following a genomic DNA, either from human or other animals.
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